UBC9 Mouse Monoclonal Antibody(C3853)

MSDS

Key features and details

Mouse monoclonal antibody to UBC9

Target: UBC9
Source/Host: Mouse
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA03465

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity . Sumoylates SHMT1 at 'Lys-38' or 'Lys-39' leading to RAN-dependent nuclear import of SHMT1 . Also sumoylates TYMS and DHFR .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal antibody to UBC9
Specificity	Recognizes endogenous levels of UBC9 protein.
Antibody Type	Primary antibody
Imnunogen	Recombinant fusion protein of human UBC9. The exact sequence is proprietary.
Purification	This antibody is purified through a protein G column.
Molecular Weight	Predicted: 18 kD; Observed: 18 kD
Form/Buffer	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18
Gene Symbol	UBE2I
Entrez Gene	7329(Human); 102641751; 22196(Mouse); 25573(Rat)
SwissProt	P63279(Human); P63280(Mouse); P63281(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of UBC9 expression in human heart (A), A431 (B), Hela (C), Jurkat (D), NIH3T3 (E), PC12 (F) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 18 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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