Type-II Cytokeratins Rabbit Monoclonal Antibody(ARA803)

Datasheet

MSDS

Type-II Cytokeratins Rabbit Monoclonal Antibody(ARA803)

Key features and details

Target: Type-II Cytokeratins
Source/Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Applications: WB,IHC,FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6590

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Size:

100μL

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Product Details

Background

There are two types of Cytokeratins: types I and II. The type I family is comprised of the acidic members, Cytokeratins 9-20, and the type II family is comprised of the basic to neutral members, Cytokeratins 1-8. The formation of intermediate filaments requires the pairing of at least one acidic and one Basic Cytokeratin. The genes encoding human type II/Basic Cytokeratins are located in a cluster on chromosome 12q. Relative to their type I partner, Basic Cytokeratins are initially expressed in differentiating epithelia. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:5000-1:10000
IHC	1:1600-1:3200
FC	1:10-1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to Type-II Cytokeratins
Antibody Type	Primary antibody
Predicted MW	51-66kDa
Immunogen	A synthetic peptide corresponding to residues aa300- 400 of human Type II Cytokeratins was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.78%.
Alternative Names	Keratin type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
Gene Symbol	KRT5
Entrez Gene	3852(Human); 110308(Mouse); 369017(Rat)
Swissprot	P13647

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-Type-II Cytokeratins antibody at 1:10,000 dilution
Predicted MW: 51-66 kDa
Observed MW: 51-66 kDa

Lane 1: A549
Lane 2: SW480
Lane 3: HaCat
Lane 4: Caco-2

Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 20s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Type-II Cytokeratins with ARA803 at 1:1,600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Overlay histogram showing Hela cells stained with ARA803 (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA803, 1:50 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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