TRAF2 Mouse Monoclonal Antibody(C3884)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to TRAF2 |
Specificity | Recognizes endogenous levels of TRAF2 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human TRAF2. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 55 kD; Observed: 55 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3 |
Gene Symbol | TRAF2 |
Entrez Gene | 7186(Human) |
SwissProt | Q12933(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of TRAF2 expression in 293 (A), Hela (B) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)
Immunofluorescent analysis of TRAF2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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