TRA-1-60 Mouse Monoclonal Antibody (TRA-1-60)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA0160
US＄ Please choose
US＄ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Embryonic stem cells have the ability to remain undifferentiated and proliferate indefinitely in vitro, while maintaining the potential to differentiate into derivatives of all three embryonic germ layers. Undifferentiated human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas and are characterized by the expression of Stage Specific Embryonic Antigens SSEA - 1 and SSEA - 3, the high molecular weight glycoproteins TRA - 1 - 60 and TRA - 1 - 81, as well as TRA - 2 - 39 and TRA - 2 - 54. Monoclonal antibodies TRA - 2 - 49 and TRA - 2 - 54 also recognize the liver isozyme of alkaline phosphatase expressed by human EC cells. TRA - 1 - 60 antigen was originally identified as a teratocarcinoma mucin - like antigen expressed on the surface of EC progenitor cells. TRA - 1 - 60 is also characterized as a tumor marker for embryonal carcinoma positive NSTGCT (nonseminomatous testicular germ cell tumors) and is co - expressed with TRA - 1 - 81 and the SSEAs on the membrane of a considerable number of stem cells.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:100-1:1000 |
IF/ICC | 1:50-1:500 |
FC | 1 µg/1×10⁶ cells |
IP | 1-2 µg/100-500 µg total protein |
Overview
Isotype | Mouse IgM |
Target Antigen | TRA-1-60 (Podocalyxin/Tumor-related antigen 1-60) |
Gene Name | PODXL |
UniProt ID | O00592 (Human) |
Entrez Gene ID | 5420 (Human) |
Molecular Weight | 200-240 kDa |
Gene Aliases | TRA-1-60, GCTM-2 antigen, Podocalyxin-like protein 1, PC, PCLP1 |
Immunogen | Human embryonal carcinoma cell line 2102Ep |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of TRA - 1 - 81 (A) and TRA - 1 - 60 (B) expression in NTERA - 2 cl.D1 whole cell lysate. Antibodies tested include TRA - 1 - 81 (TRA - 1 - 80(A) and TRA - 1 - 60 (TRA - 1 - 60)(B).
lmmunofluorescence staining of methanol-fixed NTERA2.D1 cells showing membrane localization.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
New Products
