Topoisomerase 2 alpha Mouse Monoclonal Antibody(C3007)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:250 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to Topoisomerase 2 alpha |
Specificity | Recognizes endogenous levels of Topoisomerase 2 alpha protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human Topoisomerase 2 alpha expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 174 kD; Observed: 174 kD kD |
Form/Buffer | Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme |
Gene Symbol | TOP2A |
Entrez Gene | 7153(Human); 360243(Rat) |
SwissProt | P11388(Human); P41516(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Topoisomerase 2 alpha expression in PC12 (A), Hela (B), Jurkat (C), K562 (D) whole cell lysates. (Predicted band size: 174 kD; Observed band size: 174 kD kD)
Immunofluorescent analysis of Topoisomerase 2 alpha staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-Topoisomerase 2 alpha Antibody (green) and negative control (red).
FAQs
New Products
