TOPO II Rabbit Monoclonal Antibody(ARA747)

Datasheet

MSDS

TOPO II Rabbit Monoclonal Antibody(ARA747)

Key features and details

Target: TOPO II
Source/Host: Rabbit
Reactivity: Human, Mouse
Clonality: Monoclonal
Applications: WB,IHC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6534

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Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:2000-1:5000
IHC	1:100-1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to TOPO II
Antibody Type	Primary antibody
Predicted MW	174kDa
Immunogen	A synthetic peptide corresponding to residues on the C-terminus of human TOPO II was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.22%.
Alternative Names	TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II alpha isozyme
Gene Symbol	TOP2A
Entrez Gene	7153(Human)
Swissprot	P11388

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-TOPO II antibody at 1:5,000 dilution
Predicted MW: 174 kDa
Observed MW: 174 kDa
Lane 1: Hela
Lane 2: H1975
Lane 3: JurKat
Lane 4: H441
Lane 5: PC-3
Lane 6: B16-Fo

Lysate at 10 µg per lane
2nd Ab:
G&R HRP(H+L) 1:10,000
Exposure: 120s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling TOPO-II with ARA747 at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer PH9.0.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Endometrium cancer tissue labelling TOPO-II with ARA747 at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer PH9.0.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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