TLE1/2/3/4 Rabbit Polyclonal Antibody

Datasheet

MSDS

Key features and details

Rabbit polyclonal antibody to TLE1/2/3/4

Target: TLE1/2/3/4
Source/Host: Rabbit
Reactivity: Human,Mouse,Rat,Zebrafish
Clonality: Polyclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : APA12261

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Size:

50μL 100μL

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Product Details

Background

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression . The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit polyclonal antibody to TLE1/2/3/4
Specificity	Recognizes endogenous levels of TLE1/2/3/4 protein.
Antibody Type	Primary antibody
Imnunogen	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TLE1/2/3/4. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 83; Observed: 82 kD
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	TLE1; Transducin-like enhancer protein 1; E(Sp1) homolog; Enhancer of split groucho-like protein 1; ESG1; TLE2; Transducin-like enhancer protein 2; Enhancer of split groucho-like protein 2; ESG2; TLE3; KIAA1547; Transducin-like enhancer protein 3; Enhancer of split groucho-like protein 3; ESG3; TLE4; KIAA1261; Transducin-like enhancer protein 4
Gene Symbol	TLE1; TLE2; TLE3; TLE4
Entrez Gene	7088; 7089; 7090; 7091(Human); 21885; 21886; 21887; 21888(Mouse); 84424; 25565(Rat)
SwissProt	Q04724; Q04725; Q04726; Q04727(Human); Q62440; Q9WVB2; Q08122; Q62441(Mouse); Q9JIT3; Q07141(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of TLE1/2/3/4 expression in A549 (A), K562 (B), Hela (C), NIH3T3 (D), mouse liver (E) whole cell lysates. (Predicted band size: 83; 79 kD; Observed band size: 82 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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