TIP47 Mouse Monoclonal Antibody(C3532)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to TIP47 |
Specificity | Recognizes endogenous levels of TIP47 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide of human TIP47. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 47 kD; Observed: 47 kD |
Form/Buffer | Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | M6PRBP1; TIP47; Perilipin-3; 47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; Mannose-6-phosphate receptor-binding protein 1; Placental protein 17; PP17 |
Gene Symbol | PLIN3 |
Entrez Gene | 10226(Human) |
SwissProt | O60664(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of TIP47 expression in Hela (A), K562 (B), Daudi (C) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)
Immunofluorescent analysis of TIP47 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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