TIEG1 Mouse Monoclonal Antibody (95-D)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : ARA0195
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Product Details
Background
Originally isolated from osteoblastic cells, the TGFβ - inducible early gene - 1 (TIEG1) is a Krüppel - like zinc - finger transcription factor - encoding gene which regulates cellular growth and differentiation. TIEG1 is regulated as an early response gene by TGFβ1. It is expressed in both acinar and ductular epithelial cells from exocrine pancreas and may serve as an early response gene in pancreatic cell lines. Further, overexpression of TIEG1 in TGFβ - sensitive epithelial cells induces apoptosis. TIEG1 and EGRα are expressed from alternate promoters of the same gene. Both are highly expressed in human fetal osteoblast cells. TIEG1 is additionally expressed at high levels in PBLs, spleen and colon, and at lower levels in thymus, small intestine, ovary, prostate and skeletal muscle. The nuclear TIEG2 protein, which shares significant homology with TIEG1, was originally isolated from globin - expressing human fetal erythroid cells. TIEG2 is also expressed in fetal liver. Overexpression of TIEG2 in cultured epithelial cells inhibits cellular proliferation. TIEG2 expression is upregulated by TGFβ1 and serum deprivation.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:100-1:1000 |
IF/ICC | 1:50-1:500 |
IP | 1-2 µg/100-500 µg total protein |
Overview
Isotype | Mouse IgG1 kappa light chain |
Target Antigen | TIEG1 (TGFB-inducible early growth response protein 1) |
Gene Name | KLF10 |
UniProt ID | Q13118 (Human), O88496 (Mouse), Q99MG0 (Rat) |
Entrez Gene ID | 7071 (Human), 16597 (Mouse), 114519 (Rat) |
Molecular Weight | 53 kDa (typically observed; can appear higher due to post-translational modifications) |
Gene Aliases | TIEG, TIEG-1, EGR-alpha, Krueppel-like factor 10 |
Immunogen | amino acids 381-480 mapping within an internal region of TIEG1 of human origin |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of TIEG1 expression in SW480 (A), Sol8 (B), RAW 264.7 (C) and Jurkat (D) nuclear extracts.
Immunofluorescence staining of methanol - fixed HeLa cells showing nuclear localization.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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