Tau (S305) Mouse Monoclonal Antibody(ARA976)

Datasheet

MSDS

Tau (S305) Mouse Monoclonal Antibody(ARA976)

Key features and details

Target: Tau (S305)
Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Application: ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6842

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Tau (S305) refers to a specific site on the tau protein, where a mutation in the S305N form is associated with a form of frontotemporal dementia. Research shows that phosphorylation at the S305 site normally inhibits tau aggregation, a process linked to neurodegenerative diseases like Alzheimer's and frontotemporal dementia. Mutations at this site, such as S305N, increase the proportion of a specific tau isoform (4R) and can disrupt normal brain cell function.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
ELISA	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Antibody Specificity	Recognizing Tau protein containing S305 and S356 peptides that share a sequence of HVPGGG
Isotype	IgG2b
Immunogen	Recombinant human Tau (phospho)
Cross Reactivity	Not tested
Recombinant	Not recombinant
Purification	Protein A purification
Form/Buffer	PBS, carrier-free, with optional 0.09% sodium azide
Conjugation	Unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Epitope analysis of Tau (S305) by ARA6842 by ELISA.Synthetic peptides containing indicated residues were coated onto microtiter wells. Recombinant Tau (2-244), Tau (1-368), Tau (243-368) and Tau (1-441) proteins with isoform P10636-8 or Tau (1-758) (with isoform P10636-1) were also coated. The antibody ARA6842 was applied as the primary antibody. Peroxidase-conjugated goat anti-mouse IgG was used as the secondary antibody, and signals were detected using a colorimetric assay.Result: ARA6842 recognizes Tau protein containing S305 and S356 peptides that share a sequence of HVPGGG.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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