Survivin Rabbit Monoclonal Antibody(C3194)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
IP | 1:10 - 1:50 |
Description | Rabbit monoclonal antibody to Survivin |
Specificity | Recognizes endogenous levels of Survivin protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant protein of human Survivin |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 16 kD; Observed: 16 kD |
Form/Buffer | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. |
Alternative Names | API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin |
Gene Symbol | BIRC5 |
Entrez Gene | 332(Human) |
SwissProt | O15392(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Survivin expression in Jurkat (A) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 16 kD)
Immunofluorescent analysis of Survivin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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