STAT3 (Phospho-S727) Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:100 |
IF/ICC | 1:50 - 1:200 |
IP | 1:10 - 1:100 |
Description | Rabbit polyclonal antibody to STAT3 (Phospho-S727) |
Specificity | Recognizes endogenous levels of STAT3 protein only when phosphorylated at S727. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S727 of human STAT3 protein. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 88 kD; Observed: 86 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | APRF; Signal transducer and activator of transcription 3; Acute-phase response factor |
Gene Symbol | STAT3 |
Entrez Gene | 6774(Human); 20848(Mouse); 25125(Rat) |
SwissProt | P40763(Human); P42227(Mouse); P52631(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of STAT3 (Phospho-S727) expression in HepG2 (A), A549 (B), LO2 (C), PC12 (D), 3T3L1 (E) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 86 kD)
Immunohistochemical analysis of STAT3 (Phospho-S727) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of STAT3 (Phospho-S727) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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