STAT3 (Phospho-S727) Rabbit Monoclonal Antibody(C3193)

Datasheet

MSDS

STAT3 (Phospho-S727) Rabbit Monoclonal Antibody(C3193)

Key features and details

Rabbit monoclonal antibody to STAT3 (Phospho-S727)

Target: STAT3 (Phospho-S727)
Source/Host: Rabbit
Reactivity: Human,Rat
Clonality: Monoclonal
Applications: WB, IF/ICC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA02805

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors . Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene . May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 . Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes . Activated by IL31 through IL31RA .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IF/ICC	1:50 - 1:100

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit monoclonal antibody to STAT3 (Phospho-S727)
Specificity	Recognizes endogenous levels of STAT3 protein only when phosphorylated at S727.
Antibody Type	Primary antibody
Imnunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S727 of human STAT3 protein. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 88 kD; Observed: 88 kD
Form/Buffer	Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Alternative Names	APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Gene Symbol	STAT3
Entrez Gene	6774(Human); 25125(Rat)
SwissProt	P40763(Human); P52631(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of STAT3 (Phospho-S727) expression in Jurkat (A), C6 (B), Hela (C) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 88 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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