Spectrin alpha I Rabbit Monoclonal Antibody(C1139)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:200 |
Description | Recombinant rabbit monoclonal antibody to Spectrin alpha I |
Specificity | Recognizes endogenous levels of Spectrin alpha I protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within human Spectrin alpha I. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 280 kD; Observed: 280 kD |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
Alternative Names | SPTA; Spectrin alpha chain, erythrocytic 1; Erythroid alpha-spectrin |
Gene Symbol | SPTA1 |
Entrez Gene | 6708(Human) |
SwissProt | P02549(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Spectrin alpha I expression in K562 (A) whole cell lysates. (Predicted band size: 280 kD; Observed band size: 280 kD)
Immunofluorescent analysis of Spectrin alpha I staining in MC38 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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