Smooth Muscle Actin Rabbit Monoclonal Antibody(C618)
MSDS 
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Product Details
WB | 1:500 - 1:1000 |
IHC | 1:100 - 1:500 |
IF/ICC | 1:50 - 1:200 |
Description | Recombinant rabbit monoclonal antibody to Smooth Muscle Actin |
Specificity | Recognizes endogenous levels of Smooth Muscle Actin protein. |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | Recombinant fusion protein of human Smooth Muscle Actin. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 42 kD; Observed: 43 kD |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.05% BSA and 0.01% sodium azide. |
Alternative Names | ACTSA; ACTVS; Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein |
Gene Symbol | ACTA2 |
Entrez Gene | 59(Human); 11475(Mouse); 81633(Rat) |
SwissProt | P62736(Human); P62737(Mouse); P62738(Rat) |
Western blot analysis of Smooth Muscle Actin expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 43 kD)
Immunohistochemical analysis of Smooth Muscle Actin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Smooth Muscle Actin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of Smooth Muscle Actin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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