SLBP Rabbit Monoclonal Antibody(C2008)
Datasheet 
Key features and details
Recombinant rabbit monoclonal antibody to SLBP
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : AMA01620
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Product Details
Background
RNA-binding protein involved in the histone pre-mRNA processing . Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE) . Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery . Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression . Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis . Binds to the 5' side of the stem-loop structure of histone pre-mRNAs .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
IP | 1:10 - 1:50 |
Overview
Description | Recombinant rabbit monoclonal antibody to SLBP |
Specificity | Recognizes endogenous levels of SLBP protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | Recombinant fusion protein of human SLBP. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 31 kD; Observed: 30 kD |
Form/Buffer | Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300. |
Alternative Names | HBP; Histone RNA hairpin-binding protein; Histone stem-loop-binding protein |
Gene Symbol | SLBP |
Entrez Gene | 7884(Human) |
SwissProt | Q14493(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of SLBP expression in HEK293T (A) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 30 kD)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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