SIRT1 Mouse Monoclonal Antibody (33.36)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : ARA3336
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Product Details
Background
The silent information regulator (SIR2) family of genes are highly - conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA - damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in a NAD - dependent manner, which regulates silencing at the telomeric, rDNA and silent - mating - type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1 - 7, are divided into four main branches: SIRT1 - 3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6 - 7 are class IV. SIRT1, which has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 is highly expressed in heart, brain and skeletal muscle, with low expression in lung and placenta. SIRT1 regulates the p53 - dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lys 382.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:100-1:1000 |
IHC | 1:50-1:500 |
IF/ICC | 1:50-1:500 |
ELISA | 1:30-1:3000 |
IP | 1-2 µg/100-500 µg total protein |
Overview
Isotype | IgG1 kappa light chain |
Target Antigen | SIRT1 |
Gene Name | SIRT1 |
UniProt ID | Q96EB6 (Human), Q923E4 (Mouse), Q9R160 (Rat) |
Entrez Gene ID | 23411 (Human), 93759 (Mouse), 309757 (Rat) |
Molecular Weight | 110 kDa (typically observed; theoretical mass ~81.7 kDa) |
Gene Aliases | SIR2L1, SIR2alpha, SIR2-like protein 1, NAD-dependent protein deacetylase sirtuin-1 |
Immunogen | amino acids 456-555 mapping within an internal region of SIRT1 of human origin |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of SIRT1 expression in NTERA - 2 cl.D1 (A), K - 562 (B), BJAB (C) and SUP - T1 (D) whole cell lysates.
Immunoperoxidase staining of formalin fixed, paraffin - embedded human testis tissue showing nuclear or cytoplasmic staining of cells in seminiferous ducts and cytoplasmic staining of Leydig cells.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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