Salmonella typhimurium LPS Mouse Monoclonal Antibody(ARA669)

Datasheet

MSDS

Salmonella typhimurium LPS Mouse Monoclonal Antibody(ARA669)

Key features and details

Target: Salmonella typhimurium LPS
Host: Mouse
Reactivity: Salmonella typhimurium
Clonality: Monoclonal
Application: WB, ELISA, Flow Cyt, Dot, IF/ICC, EM
Storage: -20°C

Brand:

CAT.NO. : ARA6387

US$ Please choose

Size:

50μg

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Salmonella typhimurium LPS also known as lipopolysaccharide forms a vital component of the outer membrane of Gram-negative bacteria such as Salmonella. This complex molecule consists of a lipid A domain a core oligosaccharide and an O-antigen polysaccharide chain. The lipid A component with an estimated mass of roughly 10 kDa is the main contributor to LPS endotoxin activity. In Salmonella the LPS is expressed in the bacterial cell wall providing structural integrity and defense against environmental stresses. Its composition varies among different Salmonella strains influencing the bacterium's virulence and interaction with host immune systems.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB, ELISA, Flow Cyt, Dot, IF/ICC, EM	Use at an assay dependent concentration.

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Isotype	IgG1
Host Species	Mouse
Form/Buffer	pH: 7.4 Preservative: 0.1% Sodium azide
Form	Liquid
Clonality	Monoclonal
Purification	Affinity purification Protein A
Specificity	This antibody is directed against lipopolisaccharides of Salmonella typhimurium
Light Chain Type	unknown

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Gels were (A) silver-stained or (B) Western blotted using the Salmonella typhimurium LPS Mouse Monoclonal Antibody(ARA669) as the primary antibody.Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the Salmonella typhimurium LPS Mouse Monoclonal Antibody(ARA669) on the corresponding Western blot.All lanes:Western blot - Salmonella typhimurium LPS Mouse Monoclonal Antibody(ARA669) at 1/1000 dilutionLane 1:Whole cell polysaccharides from S. Enteritidis 27655-3b at 100000000 CellsLane 2:Whole cell polysaccharides from S. Enteritidis Sal8 at 100000000 CellsLane 3:Whole cell polysaccharides from S. Typhimurium ATCC 14028s at 100000000 CellsLane 4:Whole cell polysaccharides from S. Typhimurium SL1344 at 100000000 CellsSecondaryAll lanes:AP conjugated goat anti-mouse polyclonal at 1/4000 dilution

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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