Salmonella typhimurium LPS Mouse Monoclonal Antibody(ARA669)
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
-
Brand:
CAT.NO. : ARA6387
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Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Salmonella typhimurium LPS also known as lipopolysaccharide forms a vital component of the outer membrane of Gram-negative bacteria such as Salmonella. This complex molecule consists of a lipid A domain a core oligosaccharide and an O-antigen polysaccharide chain. The lipid A component with an estimated mass of roughly 10 kDa is the main contributor to LPS endotoxin activity. In Salmonella the LPS is expressed in the bacterial cell wall providing structural integrity and defense against environmental stresses. Its composition varies among different Salmonella strains influencing the bacterium's virulence and interaction with host immune systems.
Application
|
Application |
Dilution Ratio |
|
WB, ELISA, Flow Cyt, Dot, IF/ICC, EM |
Use at an assay dependent concentration. |
Overview
|
Isotype |
IgG1 |
|
Host Species |
Mouse |
|
Storage Buffer |
pH: 7.4 Preservative: 0.1% Sodium azide |
|
Form |
Liquid |
|
Clonality |
Monoclonal |
|
Purification Method |
Affinity purification Protein A |
|
Specificity |
This antibody is directed against lipopolisaccharides of Salmonella typhimurium |
|
Light Chain Type |
unknown |
|
Concentration |
2 mg/mL The concentration of this product may be batch-dependent |
Data
Gels were (A) silver-stained or (B) Western blotted using the Salmonella typhimurium LPS Mouse Monoclonal Antibody(ARA669) as the primary antibody.
Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the Salmonella typhimurium LPS Mouse Monoclonal Antibody(ARA669) on the corresponding Western blot.
All lanes:
Western blot - Salmonella typhimurium LPS Mouse Monoclonal Antibody(ARA669) at 1/1000 dilution
Lane 1:
Whole cell polysaccharides from S. Enteritidis 27655-3b at 100000000 Cells
Lane 2:
Whole cell polysaccharides from S. Enteritidis Sal8 at 100000000 Cells
Lane 3:
Whole cell polysaccharides from S. Typhimurium ATCC 14028s at 100000000 Cells
Lane 4:
Whole cell polysaccharides from S. Typhimurium SL1344 at 100000000 Cells
Secondary
All lanes:
AP conjugated goat anti-mouse polyclonal at 1/4000 dilution
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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