RCN1 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to RCN1 |
Specificity | Recognizes endogenous levels of RCN1 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human RCN1 |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 33; Observed: 38 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | RCN; Reticulocalbin-1 |
Gene Symbol | RCN1 |
Entrez Gene | 5954(Human); 19672(Mouse) |
SwissProt | Q15293(Human); Q05186(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of RCN1 expression in A549 (A), SKOV3 (B), Hela (C) whole cell lysates. (Predicted band size: 33; 38 kD; Observed band size: 38 kD)
Immunofluorescent analysis of RCN1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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