Rat IL-1β ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 17.11 pg/ml (50 μl)
Standard Curve Range: 10.97~8000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AER0001

US$ Please choose

Size:

96T

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects.
IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0472	0.0483	0.0478
10.97	0.0572	0.0632	0.0602	0.0125
32.92	0.0723	0.0712	0.0718	0.0240
98.77	0.1191	0.1082	0.1137	0.0659
296.30	0.2382	0.2332	0.2357	0.1880
888.89	0.5772	0.5553	0.5663	0.5185
2666.67	1.3862	1.3711	1.3787	1.3309
8000.00	3.1091	3.0882	3.0987	3.0509

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	104.1	635.0	2135.6	113.2	656.1	2205.3
Standard deviation	4.9	25.3	88.1	7.5	31.5	119.1
Coefficient of variation（%）	4.7	4.0	4.1	6.6	4.8	5.4

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of rat IL-1 beta into health rat serum sample. The un-spiked serum was used as blank in these experiments.

The recovery ranged from 83% to 110% with an overall mean recovery of 92%.

Sample Values

Sample Matrix

Sample Evaluated

Range (pg/ml)

Detectable

Mean of Detectable (pg/ml)

Serum

27.9-613.9

155.1

Serum/Plasma – Thirty samples from apparently healthy rats were evaluated for the presence of IL-6 in this assay. No medical histories were available for the donors.

n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

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