E.coli HCP ELISA Kit
Instruction 
CAT.NO. : ARK6626
US$ Please choose
US$ Please choose
Size:
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Product Details
Background
Escherichia coli (E. coli) is a common host for biotech and biopharmaceutical production. Residual E. coli host cell proteins (HCPs) may impact the quality and safety of biological products, making strict detection and control indispensable.
AREX' E. coli Expression HCP Residual Detection Kit features high sensitivity and specificity for identifying HCP residues from E. coli BL21 strains. It delivers accurate and reliable results to monitor HCP levels at every stage of bioproduction.
AREX' E. coli Expression HCP Residual Detection Kit features high sensitivity and specificity for identifying HCP residues from E. coli BL21 strains. It delivers accurate and reliable results to monitor HCP levels at every stage of bioproduction.
Advantages and Features
•High Antibody Coverage: Broad antibody panel ensures full lifecycle product compatibility.
•Strong Specificity: No cross-reactivity with BSA, Protein A, HEK293 HCP, or CHO HCP.
•Superior Performance: Excellent standard curve linearity, high accuracy, good repeatability, and qualified sample spike recovery rates.
•Stable supply: Compliant with ISO 13485 quality management systems in research, development and production, with adequate raw material reserves.
•Strong Specificity: No cross-reactivity with BSA, Protein A, HEK293 HCP, or CHO HCP.
•Superior Performance: Excellent standard curve linearity, high accuracy, good repeatability, and qualified sample spike recovery rates.
•Stable supply: Compliant with ISO 13485 quality management systems in research, development and production, with adequate raw material reserves.
Overview
|
Reaction System |
2.5 hours |
|
Limit of Quantitation (LOQ) |
3 ng/mL |
|
Detection Range |
1–243 ng/mL |
|
Spike Recovery Rate |
80%–120% |
|
Limit of Detection (LOD) |
≤1 ng/mL |
|
Repeatability (Intra-Batch CV) |
≤10% |
Data
Cross-reactivity was evaluated against common process impurities, matrix components, and alternative cell-line HCPs. The assay exhibited negligible cross-reactivity, with no adverse impact on E. coli HCP quantification. Notably, the analytical recovery of all spiked samples remained consistently between 80% and 120%.
Storage
The kit should be stored at 2–8°C and protected from light, with a shelf life of 12 months. After opening, any unused components must be kept tightly sealed and stored under the same conditions (2–8°C, protected from light).
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
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