CRBN Rabbit Monoclonal Antibody(ARA644)
MSDS 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
CAT.NO. : ARA6348
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Size:
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Product Details
Background
Cereblon is a protein that in humans is encoded by the CRBN gene. The gene that encodes the cereblon protein is found on the human chromosome 3, on the short arm at position p26.3 from base pair 3,190,676 to base pair 3,221,394. CRBN orthologs are highly conserved from plants to humans. Cereblon forms an E3 ubiquitin ligase complex with damaged DNA binding protein 1 (DDB1), Cullin-4A (CUL4A), and regulator of cullins 1 (ROC1). This complex ubiquitinates a number of other proteins. Through a mechanism which has not been completely elucidated, this ubiquitination results in reduced levels of fibroblast growth factor 8 (FGF8) and fibroblast growth factor 10 (FGF10). FGF8 in turn regulates a number of developmental processes, such as limb and auditory vesicle formation. The net result is that this ubiquitin ligase complex is important for limb outgrowth in embryos. In the absence of cereblon, DDB1 forms a complex with DDB2 that functions as a DNA damage-binding protein. Furthermore, cereblon and DDB2 bind to DDB1 in a competitive manner.
Application
|
Application |
Dilution Ratio |
|
WB |
1:200 - 1:1000 |
|
IHC |
1:100 - 1:200 |
|
IP |
|
Overview
|
Antibody Type |
Recombinant Rabbit monoclonal Antibody |
|
Immunogen |
Recombinant protein within human CRBN aa 1-442 / 442. |
|
Species Reactivity |
Human, Mouse, Rat |
|
Applications |
WB, IHC, IF, IP |
|
Predicted Molecular Weight |
51 kDa |
|
Positive Control |
HEK-293 cell lysate, A375 cell lysate, A549 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, MCF7 cell lysate, mouse brain tissue lysate, rat testis tissue lysate, human testis tissue, human brain tissue. |
|
Conjugate |
Unconjugated |
|
Form |
Liquid |
|
Storage Buffer |
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
|
Isotype |
IgG |
|
Purification Method |
Protein A affinity purified. |
Data
Western blot analysis of CRBN on different lysates with CRBN Rabbit Monoclonal Antibody(ARA644) at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human testis tissue with CRBN Rabbit Monoclonal Antibody(ARA644) at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded human brain tissue with CRBN Rabbit Monoclonal Antibody(ARA644) at 1/200 dilution.
Knockdown (KD): Western blot analysis of CRBN on different lysates with CRBN Rabbit Monoclonal Antibody(ARA644) at 1/1,000 dilution.
CRBN was immunoprecipitated in 0.2mg HeLa cell lysate with CRBN Rabbit Monoclonal Antibody(ARA644) at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using CRBN Rabbit Monoclonal Antibody(ARA644) at 1/1,000 dilution.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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