RANGAP1 Rabbit Monoclonal Antibody(C1412)
MSDS 
Key features and details
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Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:200 |
Description | Recombinant rabbit monoclonal antibody to RANGAP1 |
Specificity | Recognizes endogenous levels of RANGAP1 protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within human RANGAP1 protein. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 63 kD; Observed: 64, 90 kD |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
Alternative Names | KIAA1835; SD; Ran GTPase-activating protein 1; RanGAP1 |
Gene Symbol | RANGAP1 |
Entrez Gene | 5905(Human) |
SwissProt | P46060(Human) |
Western blot analysis of RANGAP1 expression in K562 (A), MCF7 (B), Hela (C) whole cell lysates. (Predicted band size: 63 kD; Observed band size: 64, 90 kD)
Immunofluorescent analysis of RANGAP1 staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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