pVHL Mouse Monoclonal Antibody(ARA966)

Datasheet

MSDS

Key features and details

Target: pVHL
Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Application: IHC
Storage: -20°C

Brand:

CAT.NO. : ARA6829

US$ Please choose

Size:

1mL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Von Hippel–Lindau syndrome (VHL) is a dominantly inherited hereditary cancer syndrome predisposing to a variety of malignant and benign tumors of the eye, brain, spinal cord, kidney, pancreas, and adrenal glands. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. Individuals with VHL syndrome inherit one mutation in the VHL protein that causes the protein's normal function to be lost or altered. Over time, sporadic mutation in the second copy of the VHL protein can lead to carcinomas, in particular hemangioblastomas affecting the liver and kidneys, renal (and vaginal) clear cell adenocarcinomas. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This complex is involved in the ubiquitination and degradation of a hypoxia-inducible factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
IHC	1:100 - 1:300

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Description	Mouse monoclonal antibody to Von Hippel Lindau
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within human Von Hippel Lindau. The exact sequence is proprietary
Purification	The antibody was purified by immunogen affinity chromatography
Clonality	Monoclonal
Isotype	Mouse IgG2b
Form	Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide
Gene Name	VHL
Synonyms	Von Hippel-Lindau disease tumor suppressor; Protein G7; pVHL
Gene ID (Human)	7428
Protein ID (Human)	P40337

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemical analysis of Von Hippel Lindau staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of Von Hippel Lindau staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?

AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.

What development scenarios are pathology raw antibodies mainly used for?

Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.

How can I evaluate whether a raw antibody is suitable for our staining platform?

It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.

Can you provide samples for platform validation?

Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.

How should raw antibodies be paired with detection systems?

AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.

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