pTau231 Mouse Monoclonal Antibody(ARA973)

Datasheet

MSDS

pTau231 Mouse Monoclonal Antibody(ARA973)

Key features and details

Target: pTau231
Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Application: ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6839

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

pTau231 is a phosphorylated form of tau protein that is a promising biomarker for early Alzheimer's disease (AD). It is an indicator of incipient amyloid-beta pathology and can be measured in blood plasma, making it useful for early detection, differentiating AD from other neurodegenerative disorders, and identifying people for clinical trials. Elevated levels correlate with cognitive and neuropathological severity, and it can detect AD changes years before clinical symptoms appear.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
ELISA	1:250 - 1:500

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Antibody Specificity	Recognizing phosphorylated peptide (pT231)
Isotype	IgM
Immunogen	Synthetic peptide within aa200-250 of Tau-F. The exact peptide sequence is proprietary information
Cross Reactivity	Not tested
Recombinant	Not recombinant
Purification	Protein A purification
Form/Buffer	PBS, carrier-free, with optional 0.09% sodium azide
Conjugation	Unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Sandwich ELISA analysis of pTau231 by ARA6839 & ARA6860.Microtiter wells were coated with ARA6839 as the capture antibody. Synthetic peptides with different phosphorylation sites were used as the antigen. Peroxidase conjugated mouse anti-human Tau monoclonal antibody (ARA6860) was used as the detection antibody.Result: ARA6839 and ARA6860 can be used as a matched antibody pair to detect and quantify the concentration of pTau231.

Cross reactivity of ARA6839 to other peptides and recombinant proteins by ELISA.Microtiter wells were coated with various peptides and recombinant proteins.ARA6839 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgM was used as the secondary antibody.Result: ARA6839 does not cross-react with Tau peptide or recombinant proteins without any phosphorylation site.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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