pTau217 Mouse Monoclonal Antibody(ARA974)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6840
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Product Details
Background
Plasma p-tau217 is a biomarker associated with Alzheimer's disease (AD) pathology. Research has shown that plasma p-tau217 is a sensitive marker of the clinical manifestation and progression of AD, including amyloid accumulation, tau burden, brain atrophy, and physical degradation. It has been found to be a potential non-invasive biomarker of AD, with high diagnostic accuracy in differentiating AD from other neurodegenerative diseases. Plasma p-tau217 has also been associated with longitudinal increases in Aβ PET uptake and cognitive decline in Aβ-positive clinically unimpaired individuals. Additionally, higher baseline p-tau217 levels have been linked to greater future tau PET pathology burden and worse future memory performance. These findings suggest that plasma p-tau217 could serve as a minimally invasive diagnostic and prognostic biomarker for AD, with potential utility in clinical practice and trials.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
ELISA | 1:1,500- 1:2,000 |
Overview
Antibody specificity | pTau217 |
Isotype | IgG1 |
Immunogen | Synthetic peptide within aa200-250 of Tau. The exact peptide sequence is proprietary information. |
Cross reactivity | Not tested |
Recombinant | Not recombinant |
Purification | Protein A purification |
Form/Buffer | PBS, carrier-free, with optional 0.09% sodium azide |
Conjugation | Unconjugated |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Sandwich ELISA analysis of pTau217 by ARA6840 & ARA6843. Microtiter plates were coated with KAA338_16C8 as the capture antibody. Recommbinant pTau217 protein (AXP6612) was applied as the antigen. Peroxidase conjugated pTau217 Antibody (ARA6843) served as the detection antibody. Result: ARA6840 and ARA6843 can be used as a matched antibody pair to detect and quantify the concentration of pTau217.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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