pTau181 Mouse Monoclonal Antibody (ARA989)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6887
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Product Details
Background
Plasma phosphorylated tau 181 (p-tau181) has emerged as a promising biomarker for Alzheimer’s disease (AD) and related conditions. Studies show that elevated plasma p-tau181 levels are associated with increased amyloid-beta (Aβ) deposits across various brain regions in cognitively healthy adults. Furthermore, p-tau181 levels correlate with Aβ-positivity on positron emission tomography (PET), and they predict the conversion to dementia stage depending on tau-PET burden. However, p-tau181 does not appear to be directly linked to renal function. Additionally, plasma p-tau181 levels have been associated with cognitive decline in various populations, such as those with AD, mild cognitive impairment (MCI), and other neurodegenerative diseases. Moreover, p-tau181 levels have been identified as a noninvasive diagnostic and prognostic tool in clinical practice and trials.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
Sandwich ELISA | 1:250 - 1:500 |
Overview
Antibody specificity | pTau181 peptide |
Isotype | IgG2b |
Immunogen | Human MAPT peptide |
Cross reactivity | Not tested |
Recombinant | Not recombinant |
Purification | Protein A purification |
Form/Buffer | PBS, carrier-free, with optional 0.09% sodium azide |
Conjugation | Unconjugated |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Sandwich ELISA analysis of pTau181 by ARA6887 & mouse anti-human MAPT monoclonal antibody. Microtiter wells were coated with ARA6887 as the capture antibody. Phosphorylated MAPT was used as the antigen. Peroxidase conjugated mouse anti-human MAPT monoclonal antibody was used as the detection antibody. Result: ARA6887 and mouse anti-human MAPT monoclonal antibody can be used as a matched antibody pair to detect and quantify the concentration of pTau181.
Formulation
| ARA6887 | Phosphate-buffered saline (PBS), carrier-free, with optional 0.09% sodium azide |
|---|---|
| ARA6887-T5/20/50/100 | Phosphate-buffered saline (PBS) with 50% glycerol, 0.5% BSA and 0.09% sodium azide |
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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