PPAR alpha Mouse Monoclonal Antibody(C3880)
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Gene Symbol:
- Storage:
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Brand:
CAT.NO. : AMA03492
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Product Details
Background
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IHC |
1:50 - 1:200 |
|
IF/ICC |
1:10 - 1:50 |
|
FC |
1:10 - 1:50 |
Overview
|
Product Description |
Mouse monoclonal antibody to PPAR alpha |
|
Immunogen |
Recombinant fusion protein of human PPAR alpha. The exact sequence is proprietary. |
|
Purification Method |
This antibody is purified through a protein G column. |
|
Clonality |
Monoclonal |
|
Form |
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
|
Gene Symbol |
PPARA |
|
Alternative Names |
NR1C1; PPAR; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1 |
|
Gene ID (Human) |
5465 |
|
Gene ID (Mouse) |
19013 |
|
Protein ID (Human) |
Q07869 |
|
Protein ID (Mouse) |
P23204 |
Data
Western blot analysis of PPAR alpha expression in Hela (A), Jurkat (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)
Immunohistochemical analysis of PPAR alpha staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PPAR alpha staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX®Flour 488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX®Flour 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Hela cells using Anti-PPAR alpha Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX®Flour 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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