POU3F2 Mouse Monoclonal Antibody(C2914)

Datasheet

MSDS

Key features and details

Mouse monoclonal to POU3F2

Target: POU3F2
Source/Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA02526

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Transcription factor that plays a key role in neuronal differentiation . Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides . Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters . The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro . Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal to POU3F2
Specificity	Recognizes endogenous levels of POU3F2 protein
Antibody Type	Primary antibody
Imnunogen	Recombinant fusion protein of human POU3F2 expressed in E. Coli
Purification	This antibody is purified through a protein G column.
Molecular Weight	Predicted: 47 kD; Observed: 50 kD kD
Form/Buffer	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	BRN2; OCT7; OTF7; POU domain, class 3, transcription factor 2; Brain-specifIC, homeobox/POU domain protein 2; Brain-2; Brn-2; Nervous system-specifIC, octamer-binding transcription factor N-Oct-3; Octamer-binding protein 7; Oct-7; Octamer-binding transcription factor 7; OTF-7
Gene Symbol	POU3F2
Entrez Gene	5454(Human)
SwissProt	P20265(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of POU3F2 expression in Hela (A), SKNSH (B) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 50 kD kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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