PI3K p85 alpha Mouse Monoclonal Antibody（ARA820）

Datasheet

MSDS

PI3K p85 alpha Mouse Monoclonal Antibody（ARA820）

Key features and details

Target: PI3K p85 alpha
Host: Mouse
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IP, ICC/IF, IHC(P)
Storage: -20°C

Brand:

CAT.NO. : ARA6610

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:100 - 1:2000
IHC	1:50 - 1:500
IF/ICC	1:50 - 1:200
IP	-

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Species Reactivity	Human, Mouse, Rat
Isotype	Mouse / IgG1
Class	Monoclonal
Conjugate	Unconjugated
Immunogen	Recombinant protein within Human PI3-kinase p85 subunit alpha aa 19-219 / 724.
Form	Liquid
Purification	Protein A
Form/Buffer	PBS
Contains	0.09% sodium azide

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemistry analysis of PI3-kinase p85 subunit alpha in paraffin-embedded rat brain tissue. Samples were incubated with PI3-kinase p85 subunit alpha monoclonal antibody (ARA820), and followed by hematoxylin.

Immunohistochemical analysis of paraffin - embedded human lung tissue with Mouse anti - PI 3 Kinase p85 alpha antibody at 1/1,000 dilution.

Immunofluorescent analysis of PI3-kinase p85 subunit alpha in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PI3-kinase p85 subunit alpha monoclonal antibody (ARA820), followed by DAPI (blue).

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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