Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722)

Datasheet

MSDS

Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722)

Key features and details

Target: Phospho-PKA R2 (S99)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IF, IHC, IP, FC
Storage: -20°C

Brand:

CAT.NO. : ARA6507

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:1,800 - 1:2,000
IF/ICC	1:50 - 1:200
IHC	1:800 - 1:1,000
IP	1-2μg/sample
FC	1:800 - 1:1,000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic phospho-peptide corresponding to residues surrounding Ser99 of Human PKA R2 aa 82-126 / 404.
Molecular Weight	Predicted band size: 46 kDa
Positive Control	PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, human colon cancer tissue, PC-12 cells treated with 100nM Calyculin A for 30 minutes, PC-12.
Conjugation	unconjugated
Form/Buffer	1×TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG
Purification	Protein A affinity purified.

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Phospho-PKA R2 (S99) on different lysates with Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue untreated / treated with λpp with Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722).

Immunocytochemistry analysis of PC-12 cells untreated / treated with 100nM Calyculin A for 30 minutes labeling Phospho-PKA R2 (S99) with Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722).

Flow cytometric analysis of PC-12 cells labeling Phospho-PKA R2 (S99). Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722).

Phospho-PKA R2 (S99) was immunoprecipitated from 0.2 mg PC-12 cell lysate with Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722).

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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