PHLDB2 Rabbit Polyclonal Antibody
Key features and details
- Reactivity:Human, Mouse
- Host:Rabbit
- Clonality:Polyclonal
- Application:WB, IHC
- Target:PHLDB2
-
Brand:
CAT.NO. : ARA6411
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Product Details
Background
The pleckstrin homology-like domain (PHLD) class of proteins are multifunctional proteins comprised of two families of proteins, PHLDA and PHLDB, each with 3 members: PHLDA1, PHLDA2, PHDLA3, PHLDB1, PHLDB2, and PHLDB3 (PMID: 32429563). All the family members possess a pleckstrin homology (PH) domain. PHLB2, also known as LL5-beta, contains two predicted coiled-coil domains, a serine-rich region predicted to contain multiple protein kinase C phosphorylation sites in addition to a PH domain (PMID: 15851520). PHLB2 is a filamin-binding protein that is an effector of the PI3K signaling pathway (PMID: 17174070). It is a PtdIns(3,4,5)P3 (PIP3) binding protein and can translocate to the plasma membrane in a receptor and PI3K-dependant manner (PMID: 12376540). PHLB2 has also been reported as a component of the postsynaptic apparatus and a regulator of postsynaptic differentiation (PMID: 15851520).
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:10000 |
|
IHC |
1:200 - 1:1500 |
Overview
|
Application |
WB, IHC |
|
Reactivity |
Human, Mouse |
|
Immunogen |
Recombinant human PHLDB2 protein |
|
Host/Isotype |
Rabbit / IgG |
|
Antibody Type |
Polyclonal |
|
Product Type |
Antibody |
|
Full Name |
pleckstrin homology-like domain, family B, member 2 |
|
Alternative Names |
Protein LL5-beta, Pleckstrin homology-like domain family B member 2, LL5beta, LL5B |
|
Calculated Molecular Weight |
1253 aa, 142 kDa |
|
Observed Molecular Weight |
150 kDa |
|
GenBank Protein ID |
BC142678 |
|
Gene Symbol |
PHLDB2 |
|
Gene ID (NCBI) |
90102 |
|
Conjugate Type |
Unconjugated |
|
Form |
Liquid |
|
Purification Method |
Antigen affinity purification |
|
UniProt ID |
Q86SQ0 |
|
Storage Buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3 |
Data
A431 cells were subjected to SDS PAGE followed by western blot with PHLDB2 Rabbit Polyclonal Antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using PHLDB2 Rabbit Polyclonal Antibody at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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