PDHA1 Mouse Monoclonal Antibody(C3293)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
Description | Mouse monoclonal antibody to PDHA1 |
Specificity | Recognizes endogenous levels of PDHA1 protein. |
Antibody Type | Primary antibody |
Imnunogen | Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 43 kD; Observed: 43 kD |
Form/Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Alternative Names | PHE1A; Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial; PDHE1-A type I |
Gene Symbol | PDHA1 |
Entrez Gene | 5160(Human); 18597(Mouse) |
SwissProt | P08559(Human); P35486(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of PDHA1 expression in 293T (A), H1299 (B), mEsc (C), Tc1 (D) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD)
Immunofluorescent analysis of PDHA1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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