PCNA Rabbit Monoclonal Antibody(ARA811)

Datasheet

MSDS

Key features and details

Target: PCNA
Source/Host: Rabbit
Reactivity: Human, Rat, Bovine, Dog, Green Monkey, Chicken
Clonality: Monoclonal
Applications: WB,FC,IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6598

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Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Confers DNA tethering and processivity to DNA polymerases and other proteins . Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand . Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000-1:2000
FC	1:800-1:2000
IP	1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to PCNA
Antibody Type	Primary antibody
Predicted MW	29kDa
Immunogen	A synthetic peptide corresponding to residues aa100- 200 of human PCNA was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.86%.
Alternative Names	Proliferating cell nuclear antigen; PCNA; Cyclin
Gene Symbol	PCNA
Entrez Gene	5111(Human); 18538(Mouse); 25737(Rat)
Swissprot	P12004

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-PCNA antibody at 1:2,000 dilution
Predicted MW: 29 kDa
Observed MW: 29 kDa
Lane 1: A431
Lane 2: 293
Lane 3: Hela
Lane 4: HepG2
Lane 5: MCF-7
Lane 6: 3T3
Lane 7: PC-12
Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 10s

All lanes: Anti-PCNA antibody at 1:2,000 dilution
Predicted MW: 29 kDa
Observed MW: 29 kDa
Lane 1: MDCK
Lane 2: MDBK
Lane 3: COS-7
Lane 4: Chicken Heart
Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 80s

Overlay histogram showing Hela cells stained with ARA811 (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA811, 1:2,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

PCNA was immunoprecipitated from 0.4mg of Hela whole cell lysate with ARA811 at 1:50 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: ARA811 IP in Hela whole cell lysate
Lane 2: PBS instead of R704 in Hela whole cell lysate
Lane 3: Hela whole cell lysate, 10 µg (input)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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