pan-Cadherin Rabbit Monoclonal Antibody(ARB517)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6809
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R- and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells.Cadherins are expressed in a number of normal tissues, including the stomach, esophagus and kidney. Cadherins are expressed in various types of cancer tissues, including the breast cancer, cervical carcinoma and gastric cancer.Investigators consider that E-cadherin is an active suppressor of invasion and growth of many epithelial cancers. Research studies indicate that cancer cells have upregulated expression of N-cadherin and downregulated expression of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 97kDa |
Purity | ProA affinity purified IgG |
Subcellular location | Membrane and Cytoplasmic |
Swissprot ID | P12830/P19022/P22223/P55283 |
Immunogen | Synthetic peptide. |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Recommended Method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
Alternative Names | Cadherin-1 (CAM 120/80) (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) (Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3] / Cadherin-2 (CDw325) (Neural cadherin) (N-cadherin) (CD antigen CD325) / Cadherin-3 (Placental cadherin) (P-cadherin) / Cadherin-4 (Retinal cadherin) (R-CAD) (R-cadherin) |
Gene Symbol | CDH1 / CDH2 / CDH3 / CDH4 |
Entrez Gene | 999 / 1000 / 1001 / 1002 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human esophagus tissue using pan-Cadherin Rabbit Monoclonal Antibody(ARB517).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
New Products
