p-RIPK1(S166) Rabbit Monoclonal Antibody(YJY-1-5)

Datasheet

MSDS

p-RIPK1(S166) Rabbit Monoclonal Antibody(YJY-1-5)

Key features and details

Target: p-RIPK1(S166)
Source/Host: Rabbit
Reactivity: Mouse
Clonality: Monoclonal
Applications: WB,IP,IF/ICC,IHC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6605

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Receptor-interacting serine/threonine-protein kinase 1 is a serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. RIPK1 is phosphorylated at several sites within the kinase domain that are sensitive to Nec-1, including Ser14, Ser15, Ser161, and Ser166.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000-1:2000
IHC	1:100-1:500
IF/ICC	1:100-1:500
IP	1:50-1:500

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to p-RIPK1(S166)
Antibody Type	Primary antibody
Predicted MW	75kDa
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding serine 166 of Mouse RIPK1
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.97%.
Alternative Names	RIP; RIP1; Receptor-interacting serine/threonine-protein kinase 1; Cell death protein RIP; Receptor-interacting protein 1; RIP-1; Serine/threonine-protein kinase RIP
Gene Symbol	RIPK1
Entrez Gene	8737(Human); 19766(Mouse)
Swissprot	Q60855

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot and IP analysis of 661W cells, treated with combinations of the following treatments as indicated: Z-VAD (50 μM, 2 hr), mTNF-α(50 ng/ml, 2 hr), SM-164 (100 nM, 2 hr) or necrostatin-1 (Nec-1s, 30 μM, 2 hr), using BX60008 Antibody.
Western blot analysis was performed using a RIPK1 mouse Ab.
Lane 1: Lysate of untreated cells
Lane 2: Lysate of TSZ treated cells
Lane 3: Lysate of TSZ and nec-1 treated cells

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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