NKX2-5 Rabbit Polyclonal Antibody

Datasheet

MSDS

Key features and details

Rabbit polyclonal antibody to NKX2-5

Target: NKX2-5
Source/Host: Rabbit
Reactivity: Human,Mouse
Clonality: Polyclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : APA13580

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Transcription factor required for the development of the heart and the spleen . During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 . May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal . Binds to the core DNA motif of NPPA promoter . Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression . Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit polyclonal antibody to NKX2-5
Specificity	Recognizes endogenous levels of NKX2-5 protein.
Antibody Type	Primary antibody
Imnunogen	Recombinant fusion protein of human NKX2-5
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 11; Observed: 37 kD
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	CSX; NKX2.5; NKX2E; Homeobox protein Nkx-2.5; Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E
Gene Symbol	NKX2-5
Entrez Gene	1482(Human); 18091(Mouse)
SwissProt	P52952(Human); P42582(Mouse)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of NKX2-5 expression in OVCAR3 (A), SW620 (B), BT474 (C), HL60 (D), mouse heart (E), mouse spleen (F) whole cell lysates. (Predicted band size: 11; 16; 34 kD; Observed band size: 37 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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