MYPT1 (Phospho-T853) Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:100 - 1:200 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to MYPT1 (Phospho-T853) |
Specificity | Recognizes endogenous levels of MYPT1 protein only when phosphorylated at T853. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T853 of human MYPT1 protein. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 115 kD; Observed: 130 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | MBS; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit |
Gene Symbol | PPP1R12A |
Entrez Gene | 4659(Human); 17931(Mouse); 116670(Rat) |
SwissProt | O14974(Human); Q9DBR7(Mouse); Q10728(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of MYPT1 (Phospho-T853) expression in C6 (A), MCF7 (B) whole cell lysates. (Predicted band size: 115 kD; Observed band size: 130 kD)
Immunohistochemical analysis of MYPT1 (Phospho-T853) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MYPT1 (Phospho-T853) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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