MSH6 Rabbit Monoclonal Antibody(ARA741)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:2000-1:5000 |
IHC | 1:800-1:1600 |
IF/ICC | 1:400-1:1000 |
FC | 1:10-1:100 |
IP | 1:25 |
Description | Rabbit Monoclonal Antibody to MSH6 |
Antibody Type | Primary antibody |
Predicted MW | 153kDa |
Immunogen | A synthetic peptide corresponding to Ki-67 residues within aa1000-1200 of Ki-67 was used as an immunogen. |
Purification | ProA affinity purified IgG |
Form/Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.16%. |
Alternative Names | GTBP; DNA mismatch repair protein Msh6; hMSH6; G/T mismatch-binding protein; GTBP; GTMBP; MutS-alpha 160 kDa subunit; p160 |
Gene Symbol | MSH6 |
Entrez Gene | 2956(Human) |
Swissprot | P52701 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
All lanes: Anti-MSH6 antibody at 1:5,000 dilution
Predicted MW: 153 kDa
Observed MW: 163 kDa
Lane 1: Hela
Lane 2: A431
Lane 3: SW480
Lane 4: Rat testis
Lane 5: 293HEK
Lysate at 20 µg per lane
2nd Ab:
G&R HRP(H+L) 1:10,000
Exposure: 120s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling MSH6 with ARA741 at 1:500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
ARA741 staining MSH6 in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:1,000) at 4°C. An Alexa Fluor<sup>®</sup> 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor<sup>®</sup> 594-conjugated Goat Anti-Rabbit IgG (1:500).
Overlay histogram showing Hela cells stained with ARA741 (Blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA741, 1:100 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Red) was used as a control.
MSH6 was immunoprecipitated from 0.4mg of Hela whole cell lysate with ARA741 at 1:25 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: ARA741 IP in Hela whole cell lysate
Lane 2: PBS instead of ARA741 in Hela whole cell lysate
Lane 3: Hela whole cell lysate, 10 µg (input)
Exposure: 50s
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