MSH6 Rabbit Monoclonal Antibody(ARA741)

Datasheet

MSDS

Key features and details

Target: MSH6
Source/Host: Rabbit
Reactivity: Human, Rat
Clonality: Monoclonal
Applications: WB,IHC,IF/ICC,FC,IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6528

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:2000-1:5000
IHC	1:800-1:1600
IF/ICC	1:400-1:1000
FC	1:10-1:100
IP	1:25

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to MSH6
Antibody Type	Primary antibody
Predicted MW	153kDa
Immunogen	A synthetic peptide corresponding to Ki-67 residues within aa1000-1200 of Ki-67 was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.16%.
Alternative Names	GTBP; DNA mismatch repair protein Msh6; hMSH6; G/T mismatch-binding protein; GTBP; GTMBP; MutS-alpha 160 kDa subunit; p160
Gene Symbol	MSH6
Entrez Gene	2956(Human)
Swissprot	P52701

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-MSH6 antibody at 1:5,000 dilution
Predicted MW: 153 kDa
Observed MW: 163 kDa

Lane 1: Hela
Lane 2: A431
Lane 3: SW480
Lane 4: Rat testis
Lane 5: 293HEK

Lysate at 20 µg per lane
2nd Ab:
G&R HRP(H+L) 1:10,000
Exposure: 120s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling MSH6 with ARA741 at 1:500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

ARA741 staining MSH6 in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:1,000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1:500).

Overlay histogram showing Hela cells stained with ARA741 (Blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA741, 1:100 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Red) was used as a control.

MSH6 was immunoprecipitated from 0.4mg of Hela whole cell lysate with ARA741 at 1:25 dilution.
2nd Ab:
GAR HRP for IP 1:500

Lane 1: ARA741 IP in Hela whole cell lysate
Lane 2: PBS instead of ARA741 in Hela whole cell lysate
Lane 3: Hela whole cell lysate, 10 µg (input)

Exposure: 50s

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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