Mouse VEGFR3/FLT4(Vascular Endothelial Cell Growth Factor Receptor 3) ELISA Kit

Key features and details

  • Sensitivity:37.5 pg/ml
  • Detection Range:62.5 - 4000 pg/ml
  • Sample Types: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
  • Species:Mouse
  • Detection Method: Sandwich ELISA
  • Target:VEGFR3/FLT4
  • Brand:
CAT.NO. : ARE6636
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Size:
96T
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Product Details
Background
Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.
Overview

Product Description

Mouse Vascular Endothelial Growth Factor Receptor 3 (VEGFR3/Flt4) ELISA Kit

Reactivity Species

Mouse

UNIPROT ID

P35917

Application

This Mouse VEGFR3/Flt4 ELISA kit can be used for the in vitro quantitative determination of Mouse VEGFR3/Flt4 concentrations in plasma, tissue homogenates, and other biological fluids.

Detection Method

Sandwich ELISA

Detection Range

62.5 - 4000 pg/ml

Sensitivity

< 37.5 pg/ml

Intra-assay Precision

CV < 8%

Inter-assay Precision (CV)

CV < 10%

Data
Recovery

A known amount of mouse vascular endothelial growth factor receptor 3 (VEGFR3/Flt4) was spiked into the samples listed in the table below. Recovery rates were calculated by comparing the measured values to the expected amount of mouse vascular endothelial growth factor receptor 3 (VEGFR3/Flt4) in each sample.

Recovery Range (%) Mean (%)
Serum (n=5) 87-99 95
EDTA Plasma (n=5) 89-103 95
Heparin Plasma (n=5) 89-101 96
Linearity

The linearity of the kit was determined by testing samples spiked with an appropriate concentration of mouse vascular endothelial growth factor receptor 3 (VEGFR3/Flt4) and their serial dilutions. Linearity is expressed as the percentage of the measured concentration relative to the expected concentration.

Sample 1:2 1:4 1:8
Serum (n=5) 87-99% 94-105% 85-104%
EDTA Plasma (n=5) 82-101% 89-94% 85-91%
Heparin Plasma (n=5) 85-99% 80-100% 81-90%
Storage
Shipped and store at 4°C, don't cryopreserve.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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