Mouse PD1 ARExSet® antibody pair for ELISA
Protocol Booklet 
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
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Brand:
CAT.NO. : ASEM0037
US$ Please choose
US$ Please choose
Product Details
Background
PD-1 (Programmed Death-1 receptor) is a key regulator of the threshold of immune response and peripheral immune tolerance. It is expressed on activated T cells, B cells, monocytes, and dendritic cells and binds to PD-L1 or PD-L2. PD-1 ligation induces co-inhibitory signals in T cells promoting their apoptosis, anergy, and functional exhaustion. Blockade of the PD-1: PD-L1 interaction using anti-PD-1 antibodies enhances understanding of anti-tumor immunity and advances the potential for cancer immunotherapy.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.0428 | 0.0391 | 0.041 | 0.0026 | |
| 0.1280 | 0.1208 | 0.1244 | 0.0051 | 0.0835 |
| 0.2078 | 0.2249 | 0.2164 | 0.0121 | 0.1754 |
| 0.3923 | 0.3975 | 0.3949 | 0.0037 | 0.3540 |
| 0.6525 | 0.7246 | 0.6886 | 0.0510 | 0.6476 |
| 1.3220 | 1.3710 | 1.3465 | 0.0346 | 1.3056 |
| 2.3420 | 2.2560 | 2.2990 | 0.0608 | 2.2581 |
| 3.4950 | 3.3900 | 3.4425 | 0.0742 | 3.4016 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi ltered . AREX Biosciences, Catalog # DSCB01.
- Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.AREX Biosciences, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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