Mouse PD-L1 ARExSet® antibody pair for ELISA
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
-
Brand:
CAT.NO. : ASEM0036
US$ Please choose
US$ Please choose
Product Details
Background
PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0487 |
0.0472 |
0.0480 |
|
|
15.63 |
0.2260 |
0.2186 |
0.2223 |
0.2260 |
|
31.25 |
0.3659 |
0.3536 |
0.3598 |
0.3659 |
|
62.50 |
0.6048 |
0.6129 |
0.6089 |
0.6048 |
|
125.00 |
1.0800 |
1.0430 |
1.0615 |
1.0800 |
|
250.00 |
1.8400 |
1.8920 |
1.8660 |
1.8400 |
|
500.00 |
3.0960 |
2.8100 |
2.9530 |
3.0960 |
|
1000.00 |
4.1262 |
4.1028 |
4.1145 |
4.1262 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time.
- Development protocols are provided to guide further assay optimization.
- Economical alternative to complete kits.
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi
ltered . AREX Biosciences, Catalog # DSCB01. - Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution. AREX Biosciences, Catalog # DSAB01
Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01. - Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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