Mouse CXCL9/MIG ARExSet® antibody pair for ELISA
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0276 | 0.0288 | 0.0282 | |
| 15.63 | 0.1891 | 0.1990 | 0.1941 | 0.1659 |
| 31.25 | 0.3902 | 0.4016 | 0.3959 | 0.3677 |
| 62.50 | 0.6380 | 0.6202 | 0.6291 | 0.6009 |
| 125.00 | 1.0080 | 1.1180 | 1.0630 | 1.0348 |
| 250.00 | 1.4850 | 1.4880 | 1.4865 | 1.4583 |
| 500.00 | 2.3870 | 2.2530 | 2.3200 | 2.2918 |
| 1000.00 | 3.5570 | 3.2720 | 3.4145 | 3.3863 |
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.
Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
Assay Buffer: 0.5%BSA,0.05%Tween20,PBS Solution.AREX Biosciences, Catalog # DSAB01
Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.
Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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Protocol Booklet
