MINDY1 Rabbit Polyclonal Antibody

Datasheet

MSDS

Key features and details

Target: MINDY1
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Application: WB, IF/ICC, IHC
Storage: -20°C

Brand:

CAT.NO. : ARA6860

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins. Has exodeubiquitinase activity and has a preference for long polyubiquitin chains. May play a regulatory role at the level of protein turnover.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
IF/ICC	1:100 - 1:500
IHC	1:50 - 1:200
WB	1:500 - 1:2000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Reactivity	Human, Mouse, Rat
Clonality	Polyclonal
Specificity	MINDY1 Antibody detects endogenous levels of MINDY1
Conjugation	Unconjugated
Purification	The antiserum was purified by peptide affinity chromatography
Immunogen	A synthesized peptide derived from human MINDY1
UniProt	Q8N5J2 (MINDY1_HUMAN)
Gene/Base ID	MINDY1 (55793)
Protein Family	Belongs to the MINDY deubiquitinase family FAM63 subfamily

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of extracts from various samples, using MINDY1 Rabbit Polyclonal Antibody. Lane 1: Rat liver, blocked with antigen-specific peptides. Lane 2: Rat liver. Lane 3: Mouse brain. Lane 4: Hepg2 cells(heat-shock treatment). Lane 5: Hela cells(heat-shock treatment).

Immunohistochemical staining of rat heart tissue sections using MINDY1 Rabbit Polyclonal Antibody.

MINDY1 Rabbit Polyclonal Antibody staining A549 cells by IF/ICC.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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