MCM2 Mouse Monoclonal Antibody(C3441)
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Gene Symbol:
- Storage:
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Brand:
CAT.NO. : AMA03053
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Product Details
Background
MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. MCM2 has been mapped to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IHC |
1:50 - 1:200 |
|
IF/ICC |
1:50 - 1:200 |
|
FC |
1:50 - 1:200 |
|
IP |
1:10 - 1:50 |
Overview
|
Product Description |
Mouse monoclonal antibody to MCM2 |
|
Immunogen |
Purified recombinant fragment of human MCM2 expressed in E. Coli. |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Monoclonal |
|
Form |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
|
Gene Symbol |
MCM2 |
|
Alternative Names |
BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28 |
|
Gene ID (Human) |
4171 |
|
Gene ID (Mouse) |
17216 |
|
Protein ID (Human) |
P49736 |
|
Protein ID (Mouse) |
P97310 |
Data
Western blot analysis of MCM2 expression in Jurkat (A), K562 (B), NIH3T3 (C), Hela (D) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 125 kD)
Immunohistochemical analysis of MCM2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MCM2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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