Mast Cell Tryptase Rabbit Monoclonal Antibody(ARA833)

Datasheet

MSDS

Mast Cell Tryptase Rabbit Monoclonal Antibody(ARA833)

Key features and details

Target: Mast Cell Tryptase
Host: Rabbit
Reactivity: Human, Mouse
Clonality: Monoclonal
Application: WB, IP, IHC
Storage: -20°C

Brand:

CAT.NO. : ARA6647

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Mast cell chymase, known as CMA1, is a major secreted serine protease that is involved in vasoactive peptide generation, extracellular matrix degradation and regulation of gland secretion. The human chymase gene, which maps to human chromosome 14q11.2, encodes a preproenzyme with a 19-amino acid signal peptide, an acidic 2-amino acid propeptide and a 226-amino acid catalytic domain. Tryptases comprise a family of trypsin-like serine proteases that are enzymatically active as heparin-stabilized tetramers. There are four functional genes for tryptase: α I, β I, β II and γ I, which map to human chromosome 16p13.3, with β tryptases representing the main isoenzymes expressed in mast cells. Mast cell proteases are a family of rodent protein homologs to human tryptases that are specifically expressed in mast cells and may serve as highly specific markers in the analysis of mast cell heterogeneity, differentiation and function.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:1,000
IHC	1:5,000-1:10,000
IP	Use at an assay dependent concentration.
mIHC	1:3,000-1:5,000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Name	Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody
Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Recombinant protein within Human Mast Cell Tryptase aa 10-275 /275.
Species Reactivity	Human, Mouse
Validated Applications	WB, IHC-P, IP, mIHC
Molecular Weight	Predicted band size: 30 kDa
Positive Control	Human lung tissue lysates, mouse skin tissue lysates, human lung tissue, human tonsil tissue, human small intestine tissue, human prostate carcinoma tissue.
Conjugation	unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Mast Cell Tryptase antibody (ARA833) at 1/10,000 dilution.

Western blot analysis of Mast Cell Tryptase on different lysates with Rabbit anti-Mast Cell Tryptase antibody (ARA833) at 1/1,000 dilution.Lane 1: Human lung tissue lysate (40 µg/Lane) Lane 2: Mouse skin tissue lysate (40 µg/Lane)Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

New Products

hCG alpha Mouse Monoclonal Antibody(ARA629)

E.coli Residual Total RNA Detection Kit (RT-PCR)

Fibronectin Rabbit Monoclonal Antibody(C6)

Antigen Retrieval Solution II (EDTA)

Antigen Retrieval Solution I (Citrate)

Hyaluronic Acid ELISA Kit