MAS1L Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:100 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to MAS1L |
Specificity | Recognizes endogenous levels of MAS1L protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MAS1L. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 42 kD; Observed: 37 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | MRG; Mas-related G-protein coupled receptor MRG; MAS-R; MAS1-like |
Gene Symbol | MAS1L |
Entrez Gene | 116511(Human) |
SwissProt | P35410(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of MAS1L expression in Hela (A), H446 (B), mouse lung (C), mouse testis (D), rat lung (E) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 37 kD)
Immunohistochemical analysis of MAS1L staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MAS1L staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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