Mammal NF-L ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0154
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Size:
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Product Details
Background
NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0058 |
0.0061 |
0.0060 |
|
|
6.86 |
0.0192 |
0.0156 |
0.0174 |
0.0115 |
|
20.58 |
0.0364 |
0.0350 |
0.0357 |
0.0298 |
|
61.73 |
0.0947 |
0.0897 |
0.0922 |
0.0863 |
|
185.19 |
0.2678 |
0.2753 |
0.2716 |
0.2656 |
|
555.56 |
0.7753 |
0.7460 |
0.7607 |
0.7547 |
|
1666.67 |
2.0194 |
1.9554 |
1.9874 |
1.9815 |
|
5000.00 |
3.9390 |
3.9208 |
3.9299 |
3.9240 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(ng/ml) |
53.0 |
385.7 |
1612.4 |
127.7 |
547.7 |
1800.6 |
|
Standard Deviation |
2.2 |
17.4 |
76.9 |
6.7 |
25.3 |
78.4 |
|
Coefficient of Variation(%) |
4.1 |
4.5 |
4.8 |
5.2 |
4.6 |
4.4 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mammal NF-L into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 96% to 104% with an overall mean recovery of 101%.
The recovery ranged from 96% to 104% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1.18 | 25 | 0.55 |
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