LZIC Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
FC | 1:10 - 1:30 |
Description | Rabbit polyclonal antibody to LZIC |
Specificity | Recognizes endogenous levels of LZIC protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human LZIC. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 21 kD; Observed: 21 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Protein LZIC; Leucine zipper and CTNNBIP1 domain-containing protein; Leucine zipper and ICAT homologous domain-containing protein |
Gene Symbol | LZIC |
Entrez Gene | 84328(Human); 366507(Rat) |
SwissProt | Q8WZA0(Human); Q5PQN7(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of LZIC expression in K562 (A), PC12 (B) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 21 kD)
Immunofluorescent analysis of Anti-LZIC staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Hela cells using Anti-LZIC Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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