Ly6g Rabbit Monoclonal Antibody(C1960)
WB | 1:500 - 1:2000 |
IHC | 1:100 - 1:300 |
IF/ICC | 1:100 - 1:300 |
IP | 1:50 - 1:100 |
Description | Recombinant rabbit monoclonal antibody to Ly6g |
Specificity | Recognizes endogenous levels of Ly6g protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | Recombinant fusion protein of mouse Ly6g. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 14 kD; Observed: 14, 35 kD |
Form/Buffer | Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300. |
Alternative Names | Lymphocyte antigen 6G; Ly-6G; Ly-6G.1 |
Gene Symbol | Ly6g |
Entrez Gene | 546644(Mouse) |
SwissProt | P35461(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Western blot analysis of Ly6g expression in mouse kidney (A) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 14, 35 kD)

Immunohistochemical analysis of Ly6g staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with TE buffer (pH 9.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Ly6g staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with TE buffer (pH 9.0). The section was then incubated with the antibody at room temperature, and use an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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